AutoHX

Fully automated HX analysis for MS2-DIA data.

News

2024-03-22: Starting with version 3580+, the trial version of AutoHX is publicly available and no longer requires a password. Note that the trial version is limited to single-timepoint and single-protein data. To enable the full speed and functionality of AutoHX, please contact us for a license.

Description

AutoHX is a new processing option currently available in HX-DEAL. It takes advantage of MS2-DIA data to automatically assign deuteration values to peptides using fragments as redundant measurements. The MS2 redundancy allows us to assign additional certainty to both differential analysis and/or peptide kinetics analysis.

For more information, please see our Nature Communications paper below.


Paper

[Link, Data] Filandr F, Sarpe V, Raval S, Crowder DA, Khan MF, Douglas P, Coales S, Viner R, Syed A, Trainer JA, Lees-Miller SP, Schriemer DC. Automating data analysis for hydrogen/deuterium exchange mass spectrometry using data-independent acquisition methodology. Nat Com, 2200 (2024)